PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Biotechnology

The AJOL site is currently undergoing a major upgrade, and there will temporarily be some restrictions to the available functionality.
-- Users will not be able to register or log in during this period.
-- Full text (PDF) downloads of Open Access journal articles will be available as always.
-- Full text (PDF) downloads of subscription based journal articles will NOT be available
We apologise for any inconvenience caused. Please check back soon, as we will revert to usual policy as soon as possible.





Isolation of a kernel oleoyl-ACP thioesterase gene from the oil palm Elaeis guineensis Jacq.

Omorefe Asemota, Cha Tye San, Farida H Shah

Abstract


Thioesterases play a central role in determining chain lengths of fatty acids in oil storage tissues and have been isolated from a number of plant sources. While in some species enzymes that are specialized for the predominant fatty acids in the tissues examined have been found, in others, enzymes that are active over a broad range were observed. We have isolated a cDNA clone from the developing kernel of the oil palm Elaeis guineensis which encodes a thioesterase enzyme. Its highest homology was to the Brassica napus oleoyl-ACP thioesterase with which it had 72% homology at the nucleotide level, over the coding region examined, and 83% identity (90% positives) at the amino acid level.

Key Words: Elaeis guineensis, kernel, oleoyl-ACP thioesterase, cDNA.

African Jnl Biotechnology Vol.3(3) 2004: 199-201



http://dx.doi.org/10.5897/AJB2004.000-2036
AJOL African Journals Online