Prevalence of blaSHV genes in clinical isolates of Klebsiella pneumoniae at Saint Camille medical Center in Ouagadougou. Isolation of blaSHV11-like gene

  • Boukaré Zeba Laboratoire de Biochimie et de Chimie appliquée/ Enzymologie. UFR-SVT/ Université de Ouagadougou/ Burkina Faso
  • Pere Jacques Simporé Camille medical center/ Ouagadougou/ Burkina Faso
  • Odile G Nacoulma Laboratoire de Biochimie et de Chimie appliquée/ Enzymologie. UFR-SVT/ Université de Ouagadougou/ Burkina Faso
  • Jean-Marie Frere Enzymologie/ Centre d’ingenierie des protéines / Ulg/ Belgique
Keywords: &beta, --Lactamases, Klebsiella pneuminiae, bla<sub>SHV</sub> gene, Saint Camille medical centre, Ouagadougou

Abstract

Five bacterial strains (4 Klebsiella pneumoniae and 1 Escherichia coli) representative of pathogenic species and resistant to β-lactam antibiotics are investigated to isolate the genes responsible of β--lactamase activity. The use of engineering techniques enables us to show the widespread of blaSHV genes particularly in clinical isolates of K. pneumoniae. Our results highlighted an atypical blaSHV-11 gene.
Key words: β--Lactamases, Klebsiella pneuminiae, blaSHV gene, Saint Camille medical centre, Ouagadougou.
African Journal of Biotechnology Vol.3(9) 2004: 477-480
Published
2005-03-21
Issue
Vol 3 No 9 (2004)
Section
Articles
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eISSN: 1684-5315