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African Journal of Biotechnology

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Prevalence of blaSHV genes in clinical isolates of Klebsiella pneumoniae at Saint Camille medical Center in Ouagadougou. Isolation of blaSHV11-like gene

Boukaré Zeba, Pere Jacques Simporé, Odile G Nacoulma, Jean-Marie Frere

Abstract


Five bacterial strains (4 Klebsiella pneumoniae and 1 Escherichia coli) representative of pathogenic species and resistant to β-lactam antibiotics are investigated to isolate the genes responsible of β--lactamase activity. The use of engineering techniques enables us to show the widespread of blaSHV genes particularly in clinical isolates of K. pneumoniae. Our results highlighted an atypical blaSHV-11 gene.
Key words: β--Lactamases, Klebsiella pneuminiae, blaSHV gene, Saint Camille medical centre, Ouagadougou.
African Journal of Biotechnology Vol.3(9) 2004: 477-480



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