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I<i>n vitro</i> activity of three selected South African medicinal plants against human immunodeficiency virus type 1 reverse transcriptase


Pascal Obong Bessong
Chikwelu Larry Obi
Eunice Igumbor
Marie-Line Andreola
Simon Litvak

Abstract

Crude extracts of three ethnobotanically selected medicinal plants were screened for activity against two functions of human immunodeficiency type 1 reverse transcriptase. Inhibition of the RNA-dependent DNA polymerase activity was evaluated by measuring the degree of incorporation of methyl-3H thymidine triphosphate using polyadenylic acid.oligodeoxythymidylic acid as a template primer. Ribonuclease H activity was evaluated by measuring the extent of degradation of a radiolabelled RNA in an RNA/DNA hybrid by reverse transcriptase in the presence of test substance. The methanol extract of the leaves of Terminalia sericea (Combretaceae) was found to strongly inhibit the polymerase (IC50 = 7.2 µg/ml) and the ribonuclease H (IC50 = 8.1 µg/ml) activities. Isolation and characterization of a possible active molecule is warranted.

Key Words: HIV-1 reverse transcriptase; inhibition; crude extracts; medicinal plants; Terminalia sericea; South Africa.

African Journal of Biotechnology Vol.3(10) 2004: 555-559

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eISSN: 1684-5315