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African Journal of Biotechnology

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Mutation induced enhanced biosynthesis of lipase

KVVSN Bapiraju, P Sujatha, P Ellaiah, T Ramana

Abstract


The purpose of the present investigation is to enhance production of biomedically important enzyme lipase by subjecting the indigenous lipase producing strain Rhizopus sp. BTS-24 to improvement by natural selection and random mutagenesis (UV and N-methyl-N'-nitro-N-nitroso guanidine, NTG). The isolation of mutants and the lipolytic activity of selected mutants were described. The best natural selectant BTNS12 showed 110% higher lipase activity than the wild strain (BTS-24). The lipase yield of the best UV mutant BTUV3 was 164% higher than the parent strain (BTNS12) and 180% times higher than the wild strain (BTS-24). Also, the lipase yield of the best NTG mutant BTNT2 was 133 % higher than the parent strain (BTUV3) and 232% higher than the wild strain (BTS-24). The results indicated that UV and NTG were effective mutagenic agents for strain improvement of Rhizopus sp. BTS-24 for enhanced lipase productivity.
Key Words: Lipase, Rhizopus, UV, NTG.
African Journal of Biotechnology Vol.3(11) 2004: 618-621



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