Study on transformation of cowpea trypsin inhibitor gene into cauliflower (Brassica oleracea L. var. botrytis)

  • Lv Lingling Southern Subtropical Crop Research Institute, Zhan Jiang, Guang Dong , 524091
  • Lei Jianjun South China Agricultural University, Guang Zhou, Guang Dong , 510642
  • Song Ming Southwest Agricultural University, Chong Qing, 400716
  • Li Liyun Agriculture & Industry & Commerce Secondary professional School, Zhan Jiang, Guang Dong , 524091
  • Cao Bihao
Keywords: cauliflower (Brassica oleracea L. var. botrytis) agrobacterium -mediated genetic transformation, CpTI gene, transgenic plant.

Abstract

Cowpea Trypsin Inhibitor (CpTI) gene was transferred into cauliflower by agrobacterium-mediated transformation method, and 14 transgenic cauliflower plants were obtained. Cotyledons and hypocotyls were used as explants. The putative transformants were assayed by PCR and Southern blotting analysis. The results indicated that CpTI gene was transferred into cauliflower successfully. The result of preliminary insect-resistant assay showed that the transgenic plants were more resistant to Pieris rapae than non-transgenic plants.
Key Words:
African Journal of Biotechnology Vol.4(1) 2005: 45-49
Published
2005-03-24
Issue
Vol. 4 No. 1 (2005)
Section
Articles
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eISSN: 1684-5315