Studies on lysine production by Bacillus megaterium

  • I A Ekwealor Department of Applied Microbiology and Brewing Nnamdi Azikiwe University, Awka Anambra State Nigeria.
  • J A N Obeta Department of Microbiology University of Nigeria Nsukka, Enugu State, Nigeria
Keywords: Lysine, amino acid, Bacillus megaterium, fermentation, penicillin

Abstract

A Lysine-producing strain recovered from soil was found to produce large amount of the amino acid. The bacterium identified as Bacillus megaterium SP 14 accumulated a lysine yield of 3.56 mg/ml in a broth culture in 96 h. Fermentation experiments show that 8.0% (w/v) glucose and 4.0% (w/v) ammonium chloride used as sources of carbon and nitrogen, respectively, in a medium/fermenter volume ratio of 25.0%, influenced accumulation of the amino acid. Amino acids other than the aspartate family at 0.01% (w/v) stimulated growth and improved lysine yield. Addition of 0.01 unit/ml penicillin to the fermentation medium, immediately after inoculation, stimulated growth and appreciably enhanced lysine accumulation.

African Journal of Biotechnology Vol. 4 (7), pp. 633-638, 2005
Published
2005-08-19
Section
Articles

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eISSN: 1684-5315