In this study, aqueous methanol and n-butanol extracts of aerial parts of Solanum trilobatum L. (Solanaceae) were tested for antimicrobial activity by disc diffusion method. From the results, it was found that extracts from leaves, flowers, stem and fruits revealed antimicrobial activity against Gram (+) and Gram (-) bacteria. Maximal antibacterial activity was seen against Klebsiella with aqueous extract whereas methanol extract of stem showed maximal activity against Staphylococcus aureus. The
Minimum Inhibitory Concentration (MIC) exhibited by S. trilobatum aqueous extracts against tested organisms ranged between 0.06-0.5 mg/ml. Presence of tannins, saponins, flavanoides, phenolic
compounds, cardiac glycosides and carbohydrates indicates S. trilobatum, is one of the potential medicinal plant for therapeutic use.