Synergistic effects of squalene and polyunsaturated fatty acid concentrate on lipid peroxidation and antioxidant status in isoprenaline-induced myocardial infarction in rats

  • N Dhandapani
  • B Ganesan
  • R Anandan
  • R Jeyakumar
  • D Rajaprabhu
  • RA Ezhilan
Keywords: Squalene, PUFA, isoprenaline, myocardial infarction, lipid peroxidation, antioxidant defense system

Abstract

We have studied the synergistic effects of squalene and polyunsaturated fatty acids (PUFA concentrate) on isoprenaline-induced infarction in rats with respect to changes in the levels of plasma diagnostic marker enzymes and myocardial antioxidant defense system. Intraperitoneal injection of isoprenaline caused a significant elevation in the levels of diagnostic marker enzymes; alanine
aminotranferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) in plasma of experimental rats. There was a significant rise in the level of lipid
peroxidation with concomitant decline in the level of reduced glutathione (GSH) and in the activities of glutathione-dependent antioxidant enzymes; glutathione peroxidase (GPX) and glutathione-Stransferase (GST), and antiperoxidative enzymes; superoxide dismutase (SOD) and catalase (CAT) in heart tissue. Combined supplementation of squalene and PUFA concentrate significantly prevented the isoprenaline-induced elevations in the levels of diagnostic marker enzymes in plasma of experimental groups of rats. A tendency to counteract the isoprenaline induced lipid peroxidation was also noticed. Their combined administration maintained the level of GSH and the activities of glutathione-dependent antioxidant enzymes and antiperoxidative enzymes at near normalcy. The results of the present study indicated that the combined administration of squalene and PUFA concentrate exerted significantly better cardio-protection against isoprenaline-intoxication as compared to that of per second
supplementation.
Published
2010-07-26
Section
Articles

Journal Identifiers


eISSN: 1684-5315