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African Journal of Biotechnology

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Agronomic performance of locally adapted sweet potato (Ipomoea batatas (L) Lam.) cultivars derived from tissue culture regenerated plants

JN Oggema, MG Kinyua, JP Ouma, JO Owuoche

Abstract


Tissue culture techniques have opened a new frontier in agricultural science by addressing food security and agricultural production issues. A study was conducted to compare growth and yield characteristics between the tissue culture regenerated and conventionally propagated sweet potato cultivars. Five locally adapted sweet potato cultivars Mugande, SPK004, Kemb10, Japon tresmesino and Zapallo were regenerated in vitro by the methods of indirect and direct embryogenesis and grown under field conditions in a RCBD replicated three times. Significant (P<0.05) interaction was detected
between the test cultivars and regeneration method for the growth and yield variables. The highest tuber numbers and marketable yield was recorded with Zapallo. Conventional propagation method gave
highest growth rates however the difference in yield between the conventional propagation and tissue culture regenerated plants did not vary significantly (P<0.05). Likewise, virus detection for SPFMV by
ELISA established that field plants had a higher virus titre compared to the tissue culture regenerated plants.



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