PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Biotechnology

Log in or Register to get access to full text downloads.

Remember me or Register



Influence of osmotic and metal stresses on nitrogenase activity of cyanobacteria ýsolated from paddy fields

G Okmen, G Donmez, S Donmez

Abstract


Samples were collected from paddy fields in Corum-Turk.ye. Nitrogen-free BG-11 medium was used for isolation of nitrogen fixing cyanobacteria. Acetylene reduction technique was used to determine the effects of different chemical agents on the nitrogenase activities of the cyanobacteria, which were identified at the genus level. Nostoc showed the highest nitrogenase activity (0.09 ethylene ƒÊl/mg.h) at 50 mM salt concentration. At 60 mM sucrose concentration, Nostoc showed the highest nitrogenase activity (0.08 ethylene ƒÊl/mg.h). The highest tolerances for the metals were present in Anabaena (0.006 ethylene ƒÊl/mg.h) for iron (20 ppm), Nodularia 0.1 ethylene ƒÊl/mg.h (for manganese 20 ppm) and Nostoc 0.96 ethylene ƒÊl/mg.h (for zinc 5 ppm) .



http://dx.doi.org/10.5897/AJB2007.000-2270
AJOL African Journals Online