African Journal of Biotechnology

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Phosphate uptake kinetics and its regulation in N2- fixing cyanobacterium Anabaena oryzae Fritsch under salt stress

SK Singh, V Pandey, KD Pandey


Phosphorous (P) starved cells of the cyanobacterium Anabaena oryzae showed higher phosphate uptake rates than P-sufficient cells. The P-uptake obeyed saturation kinetics. The Km value for Pdeficient
cells was lower (54.34 mM) than P-sufficient cells (82.64 mM) while Vmax was higher in Pdeficient and lower in P-sufficient cells. Salinity (NaCl) stimulated phosphate uptake significantly in the
cyanobacterium which is followed by greater amount of P-accumulation in the form of polyphosphate bodies. Inhibition of P-uptake in P-deficient cells was 45% in dark grown compared to light grown cells.
P-uptake was inhibited 52 and 85% in culture treated with 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU; 10 mM) and carbonyl cyanide m-chloro phenylhydrazone (CCCP; 100 mM), respectively,
suggesting that energy for uptake could be derived from oxidative phosphorylation and photophosphorelation.

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