In vitro clonal propagation of the neem tree (Azadirachta indica A. Juss.)
AbstractA study was conducted with root and shoot tip explants of neem to develop an efficient protocol of regeneration. Shoot tips and root tips from 10 - 20 days old seedlings of neem were cultured on Murashige and Skoog’s (MS) medium supplemented with different concentrations and combinations of BAP (0.0, 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 mg l-1) and NAA (0.0 and 0.05 mg l-1). Shoot buds were initiated from the both explants. The highest percentage of regeneration was found from shoot tip but the highest average number of shoots/explants was found from root tip explants. The regenerated shoots were further subcultured and later could be rooted on
a medium supplemented with different concentrations and combinations of IBA and IAA and complete plants could be obtained. The rooted plants were transplanted to pots for hardening.