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African Journal of Biotechnology

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Evaluation of the shoot regeneration response in tissue culture of pigeonpea (Cajanus cajan [L.] Millsp.) varieties adapted to eastern and southern Africa

S de Villiers, Q Emongor, R Njeri, E Gwata, D Hoisington, I Njagi, S Silim, K Sharma

Abstract


Seven varieties of pigeonpea (Cajanus cajan [L.] Millsp.) of varying growth durations and adapted to a wide range of environments across eastern and southern Africa were evaluated for their shoot regeneration response in tissue culture. On a standardized shoot regeneration medium, the short
duration varieties (ICPV 88091 and ICPV 86012) generally responded faster and better than the medium duration (ICEAP 00554 and ICEAP 00557) and long duration (ICEAP 00020, ICEAP 00040 and ICEAP
00053) varieties. However, all the tested varieties produced healthy rooted plants in vitro that could be transferred to the greenhouse where they exhibited normal growth, flowering and viable seed set. This
study established the basis for genetic engineering of African pigeonpea varieties.



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