Article Sidebar
Keywords:
Chlorophytum borivilianum bio-inoculants bio-hardening genetic fidelity RAPD analysis.
Article Details
Issue
Section
Articles
Copyright for articles published in this journal is retained by the journal.
Main Article Content
Biological hardening and genetic fidelity testing of micro-cloned progeny of <i>Chlorophytum borivilianum</i> Sant. et Fernand.
Abstract
The micro-cloned plantlets of Chlorophytum borivilianum registered more than 95% establishment in soil following treatment with various bio-inoculants namely; Glomus aggregatum, Trichoderma harazianum and Piriformospora indica whereas Azospirullum sp. (CIM-azo) and Actinomycetes sp. (CIM-actin) showed only up to 85% plantlet establishment. The un-rooted shoots were also treated with
these bio-inoculants, for in vivo root induction and increased survival rate/establishment frequency when transferred to soil. The un-rooted shoots also showed in vivo rooting (50%) when treated with
mycorrhiza Glomus aggregatum (VAM) and Trichoderma harazianum. The genetic fidelity testing of micro-cloned, bio-hardened progeny based on a RAPD analysis using 40 random decamer DNA primers indicated a strong uniformity in relation to the parent genotype.
these bio-inoculants, for in vivo root induction and increased survival rate/establishment frequency when transferred to soil. The un-rooted shoots also showed in vivo rooting (50%) when treated with
mycorrhiza Glomus aggregatum (VAM) and Trichoderma harazianum. The genetic fidelity testing of micro-cloned, bio-hardened progeny based on a RAPD analysis using 40 random decamer DNA primers indicated a strong uniformity in relation to the parent genotype.
