Preliminary studies on the production of endo-1,4-β–Dglucanases activity produced by Enterobacter cloacae

  • AJ Sami
  • M Awais
  • AR Shakoori

Abstract

We report the production and characterization of endo-β-1, 4-glucanase from isolated phytopathogenic bacterium Enterobacter cloacae. The bacterium was grown on different carbon sources including
carboxymethyl cellulose (CMC) and 2% Avicel, for the production of endo-1, 4-β –D-glucanases enzyme. E. cloacae produced maximum levels of cellulases after 96 h of fermentation. Higher levels of
endoglucanases were produced when microbe was grown on CMC. Endo-1, 4- β-D- glucanase had optimum pH and temperature of 5.8 and 40°C. The enzyme was inactivated by calcium chloride and a reducing agent β-mercaptoethanol.
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