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ochratoxins. Toxins were extracted and analysed by high performance liquid chromatography and fluorometry. Twenty-one of the twenty-three samples were contaminated by Aspergillus flavus which cooccurred
with A. parasiticus in two samples, A. tamarii in seven and Fusarium moniliforme in one. Rhizopus stolonifer, R. oryzae and yeast were also isolated. Aflatoxins were detected in seventeen samples, fumonisin in six and zearalenone in three. Aflatoxin levels ranged between 0.8 ± 0.2 and 156 ± 8 ìg/kg (ppb), zearalenone between 100 ± 10.5 and 165 000 ± 200 ìg/kg and fumonisin B1 between 15 ± 3.0 and 5 900 ± 40
ìg/kg. Ochratoxins were not detected in any of the samples. In most countries worldwide, legislated levels for aflatoxins and patulin are 20 ìg/kg and 50 ìg/kg, respectively, for human foods. Fumonisins,
zearalenone and other toxins are not legislated in most of the countries. Ten of the feeds contain toxin levels above legislated limits (for Canada and the USA) and guidelines set by other countries. The results of
this study highlight the need for mycotoxin legislation in the animal feed industry.