Cloning and functional characterization of a class III chitinase gene from grapevine: Inhibition of fungal growth by recombinant VvChiF III

  • SA Salami
  • A Ebadi
  • MR Naghavi
  • IB Dry
Keywords: Vitis, chitinase genes, recombinant protein, antifungal activity, SNPs

Abstract

To characterize the structure and function of chitinase genes, a class III chitinase gene (VvChiF III) was isolated from Vitis vinifera cv. Flame seedless. The VvChiF III open reading frame comprised 894
nucleotides with no introns and encoded a protein of 297 amino acids. The amino acid sequence encoded by VvChiF III showed a high identity to that of a class III chitinase isolated from V. vinifera cv. Koshu and to other acidic chitinase. Analysis of the VvChiF III amino acid sequence showed that this gene corresponds to the Glyco-hydro-18 super family that consisting of a signal peptide with the length of 25 amino acids. Purified VvChiF III showed chitinase activity toward the soluble substrate,
glycolchitin and antifungal activity against Botrytis cinerea.
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eISSN: 1684-5315