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African Journal of Biotechnology

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Studies on isolation and partial purification of lysozyme from egg white of the lovebird (Agapornis species)

R Saravanan, A Shanmugam, P Ashok, DS Kumar, K Anand, A Suman, FR Devadoss

Abstract


Lysozyme was extracted from the egg white of the eggs of lovebird (Agapornis sp.) and partially purified using the dialysis membrane. The amount of lysozyme extracted was determined through absorbance
assays using Micrococcus lysodeikticus cell walls as substrate. The total protein content in the crude (0.5 mg/ml) and partially purified (0.01 mg/ml) enzyme extract was estimated. The presence of lysozyme enzyme in the egg white was confirmed and its molecular weight (14.3 kDa) was  determined through SDS-PAGE.



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