Pullulan production from coconut by-products by Aureobasidium pullulans

  • K Thirumavalavan
  • TR Manikkadan
  • R Dhanasekar
Keywords: Pullulan, Aureobasidium pullulans, coconut water, coconut milk, characterization, FT-IR.


The production of pullulan from coconut water and coconut milk by Aureobasidium pullulans was investigated. The highest production of pullulan in batch fermentation with coconut water yield of 38.3
(g/L) and coconut milk yield 58.0 (g/L). The effect of initial pH, fermentation time, and additional nitrogen source was investigated. The highest pullulan concentration of 58.0 g/L was obtained for a fermentation
of period of 144 h with the initial substrate concentration equivalent to 50 g/L and pH 7. The chemical structure of the pullulan synthesized from coconut by-products exhibited was similar when it was compared using FT-IR spectroscopy with that of pullulan available commercially.

Journal Identifiers

eISSN: 1684-5315