Laboratory scale production of the human recombinant iduronate 2-sulfate sulfatase-Like from Pichia pastoris

  • HA Córdoba-Ruiz
  • RA Poutou-Piñales
  • OY Echeverri-Peña
  • NA Algecira-Enciso
  • P Landázuri
  • H Sáenz
  • LA Barrera-Avellaneda
Keywords: Pichia pastoris, iduronate 2-sulfate sulfatase, monod model, Hunter syndrome, methanol induction.

Abstract

Clone IDS28 of the yeast Pichia pastoris expressing the human iduronate 2-sulfate sulfatase-Like (hIDSLike) was employed for low-scale production of the recombinant enzyme in a saline culture media without phosphate. The biological activity found was between 7.3 and 29.5 nmol h-1 mg-1 of total protein. It is about 1.73 to 7 times higher than the result obtained with the same clone in shake flask culture.
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eISSN: 1684-5315