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African Journal of Biotechnology

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Genetic variability of Artemisia capillaris (Wormwood capillary) by random amplified polymorphic DNA (RAPD) in Terengganu State, Malaysia

SMZ Hasan, MSB Shafie, RM Shah

Abstract


The genetic variability among individuals of Artemisia capillaris from state of Terengganu, Malaysia was examined by using the random amplified polymorphic DNA (RAPD) technique. The samples were collected from differences regional in Terengganu State. The genomic DNA was extracted from the samples leaves. Fifthty-seven oligonucleotide primers were screened and ten primers were selected (OPA 04, OPA 09, OPA 16, OPA 17, OPA 18, OPG 03, OPG 05, OPG 09, OPG 15 and 391) to amplify DNA
from five samples of A. capillaris. A total of 335 RAPD fragments (RAPDs) with all polymorphic fragments (100%) with size ranging from 150 – 3000 bp were scored from the population. Genetic distance for samples ranges from 0.0000 to 0.2600.



AJOL African Journals Online