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Eukaryotic translation initiation factor 5A of wheat: Identification, phylogeny and expression
Abstract
In the present study, we report on the characterization of a full-length cDNA clone (TaeIF5A-1) and as well as two genomic sequences (TaeIF5A-2 and TaeIF5A-3) encoding eIF5A in wheat (Triticum
aestivum). In addition, 9 partial DNA sequences of eIF5A gene were also isolated from different species of triticeae tribe. Phylogenetic analysis of eIF5A coding genes in general revealed that TaeIF5A-1 represents ancestral types of the gene family in plant. Chromosome location analysis shows that TaeIF5A-2 was located on the chromosome 2BL. The mRNA level of TaeIF5A-1 has spatial and temporal difference, indicating it mainly plays important role in leaf development.
aestivum). In addition, 9 partial DNA sequences of eIF5A gene were also isolated from different species of triticeae tribe. Phylogenetic analysis of eIF5A coding genes in general revealed that TaeIF5A-1 represents ancestral types of the gene family in plant. Chromosome location analysis shows that TaeIF5A-2 was located on the chromosome 2BL. The mRNA level of TaeIF5A-1 has spatial and temporal difference, indicating it mainly plays important role in leaf development.
