Microbial biocatalytic preparation of 2-furoic acid by oxidation of 2-furfuryl alcohol and 2-furanaldehyde with Nocardia corallina

  • HI Pérez
  • N Manjarrez
  • A Solís
  • H Luna
  • MA Ramírez
  • J Cassani
Keywords: Biocatalyst, 2-furoic acid, 2-furanaldehyde, 2-furfuryl alcohol, Nocardia corallina, oxidation.


The preparation of 2-furoic acid via oxidation of 2-furfuryl alcohol and 2-furanaldehyde was studied by two procedures using whole cells of Nocardia corallina B-276. We describe a comparison using a batch
cultivation (3-L) versus resting cells in potassium phosphate buffer (0.1 M, pH 7.0). In the 3-L bioreactor, 2-furfuryl alcohol (1.35 g/L) was biotransformed after 24 h, to the acid with 81% yield. The bioconversion
of 2-furfuryl alcohol using resting cells leads to and almost quantitative yield (98%) of 2-furoic acid in 21 h. 2-furanaldehyde was also oxidized to the 2-furoic acid in 88% yield in 8 h, the ratio substrate:cells
was 1:3.5 (w/w). This method was scaled up to 9 g/L of 2-furanaldehyde.

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eISSN: 1684-5315