Analysis of genetic relationships of mulberry (Morus L.) germplasm using sequence-related amplified polymorphism (SRAP) markers

Abstract

Sequence-related amplified polymorphism (SRAP) is a novel molecular marker technique designed to amplify open reading frames (ORFs). Here, for the first time, SRAP was used to identify mulberry germplasm. Twenty-three mulberry accessions were screened using SRAP techniques, with 12 primer combinations selected for their reproducibility and polymorphism. Out of 83 PCR fragments scored, 59 (71.1%) were polymorphic, with an average of 4.9 polymorphic bands and 6.9 bands per primer combination. Mean gene diversity and PIC were 0.1611 and 0.1353, respectively. The genetic similarity coefficient ranged from 0.6905 to 0.9524, with an average of 0.8330. The phylogenetic tree was obtained using the UPGMA method using the total number of amplified SRAP fragments. The results from cluster analysis were in general agreement with our morphologic classification.