An effective method of disinfection protocol and micropropagation with an enhanced survival rate of explants and reduced phenol induced browning in strawberry was developed. The survival rate of three genotypes was between 89.2 - 100%. Shoot tip were able to develop into plantlet on a hormone-free MS medium when cultured under dim light (500 lux). Two media, M1 and M2 were chosen to compare the effect on shoot multiplication of three genotypes. The shoot number per responding explants was between 5 - 8 and 11 - 14 after induction.