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African Journal of Biotechnology

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The single-cell gel electrophoresis assay to determine apoptosis induced by siRNA in Colo 320 cells

H Hao, F lei, Y Nancai, S Wen, Q Xiaojuan

Abstract


The aim of the present study was to determine if the pattern of DNA fragmentation determined by the single cell gel electrophoresis assay can be used to determine apoptosis induced by siRNA in Colo 320 cells. When the frequency of appearance of apoptotic cells following was observed over a period of time, there was a significant increase in appearance of apoptosis when using single cell gel electrophoresis assay. The present report demonstrates that the characteristic pattern of apoptotic comets detected by the comet assay corresponds to cells undergoing apoptosis.



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