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African Journal of Biotechnology

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Studies on antioxidant and antiradical activities of Dolichos biflorus seed extract

B Hazra, R Sarkar, S Mandal, S Biswas, N Mandal

Abstract


The antioxidant potential of 70% methanolic extract ofDolichos biflorus seeds was assessed using different tests including total antioxidant activity, hydroxyl radical scavenging, superoxide radical scavenging, nitric oxide radical scavenging, hypochlorous acid scavenging, reducing power and lipid peroxidation. The total phenolic and flavonoid contents were also evaluated. The extract was found to be an antioxidant with a trolox equivalent antioxidant capacity (TEAC) value 0.28 ± 0.006, the extract has shown its scavenging activity for different radicals and 184.55 ± 7.08, 114.14 ± 6.85, 74.77 ± 1.73, 287.9 ± 8.56 μg/ml were determined as IC50 value for hydroxyl, superoxide, nitric oxide and hypochlorous acid respectively. The extract has shown moderate inhibition of lipid peroxidation with IC50 128.63 ± 3.44  Dolichos biflorus seeds was assessed using different tests including total antioxidant activity, hydroxyl radical scavenging, superoxide radical scavenging, nitric oxide radical scavenging, hypochlorous acid scavenging, reducing power and lipid peroxidation. The total phenolic and flavonoid contents were also evaluated. The extract was found to be an antioxidant with a trolox equivalent antioxidant capacity (TEAC) value 0.28 ± 0.006, the extract has shown its scavenging activity for different radicals and 184.55 ± 7.08, 114.14 ± 6.85, 74.77 ± 1.73, 287.9 ± 8.56 μg/ml were determined as IC50 value for hydroxyl, superoxide, nitric oxide and hypochlorous acid respectively. The extract has shown moderate inhibition of lipid peroxidation with IC50 128.63 ± 3.44 Dolichos biflorus seeds was assessed using different tests including total antioxidant activity, hydroxyl radical scavenging, superoxide radical scavenging, nitric oxide radical scavenging, hypochlorous acid scavenging, reducing power and lipid peroxidation. The total phenolic and flavonoid contents were also evaluated. The extract was found to be an antioxidant with a trolox equivalent antioxidant capacity (TEAC) value 0.28 ± 0.006, the extract has shown its scavenging activity for different radicals and 184.55 ± 7.08, 114.14 ± 6.85, 74.77 ± 1.73, 287.9 ± 8.56 μg/ml were determined as IC50 value for hydroxyl, superoxide, nitric oxide and hypochlorous acid respectively. The extract has shown moderate inhibition of lipid peroxidation with IC50 128.63 ± 3.44 g/ml. It was also observed that the plant extract (100 mg) has 44.67 ± 0.004 mg/ml gallic acid equivalent phenolic and 72.0 ± 0.008 mg/ml quercetin equivalent flavonoid content. The present results demonstrate D. biflorus seeds as a potential source of natural antioxidant.



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