Agrobacterium-mediated transient expression system in banana immature fruits

  • K Matsumoto
  • G Cabral
  • J Teixeira
  • D Monte
Keywords: Agrobacterium tumefaciens, co-cultivation, GUS, Musa, promoter.

Abstract

Transient expression assays are useful for saving time in studies of gene function, particularly ones expressed in fruit. Transient expression system of a reporter gene in immature fruit of banana was developed.

Agrobacterium tumefaciens, whose plasmid contains an intron-containing

 

Agrobacterium tumefaciens, whose plasmid contains an intron-containing

Agrobacterium tumefaciens, whose plasmid contains an intron-containing

-glucuronidase (gusA) gene under regulatory control of the CaMV 35S promoter, was vacuum-infiltrated into sliced fruits and co-cultured. GUS histochemical assay was performed three days after co-cultivation, and a high level of GUS expression was observed. This transient expression system is useful for routine transient assays to validate genes expressed in banana fruit.
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eISSN: 1684-5315