Effects of NaCl treatment on the antioxidant enzymes of oilseed rape (Brassica napus L.) seedlings

  • D Qi-lin
  • C Chen
  • F Bin
  • L Ting-ting
  • T Xia
  • G Yuan-ya
  • S Ying-kun
  • W Jin
  • D Shi-zhang
Keywords: Salt stress, Brassica napus L., anti-oxidant enzymes, quantitative real-time PCR.

Abstract

The effects of NaCl treatment on the activity of antioxidant enzymes in leaves of oilseed rape seedlings (Brassica napus L.) were studied. The results showed that the relative water content from leaves of oilseed rape seedlings was gradually decreased and the electronic conductivity was increased during 0 - 24 h under 200 mmol.l-1 NaCl treatments. The activity of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) was gradually increased during 0 - 24 h under 200 mmol.l-1 NaCl stress. After 24 h, the activities of these antioxidases were maximum and subsequently decreased. Quantitative realtime PCR analysis revealed that they were salt-inducible genes and their transcript levels were gradually increased during 0 - 24 h and most abundant after 24 h treatment with 200 mmol.l-1 sodium chloride. Therefore, these results from above indicated that the expressions of POD, SOD and CAT genes were induced by NaCl; the activities of POD, SOD and CAT were increased, which enhanced the tolerance of oilseed oilseed rape plants against NaCl stress.
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eISSN: 1684-5315