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concentration. The optimum growth temperature was 37°C. The organism could degrade completely within 120 h when initial concentration was less than 600 mg/l. Enzyme assay through cell free extract showed the presence of catechol-1,2-dioxygenase. The specific activity was 0.146 ìmol/min/mg protein. However higher concentrations of phenol in the medium showed a negative effect on the growth of the bacterium. Hence X. flavus can be effectively used for bioremediation of phenol-contaminated sites.