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African Journal of Biotechnology

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Investigation of nasal colonization of health care workers by methicillin-resistant Staphylococcus aureus with using new generation real-time PCR assay: Discussing of risks

Y Zer, I Karaoglan, M Namýduru, I Balci, ID Karagoz, M Ozaslan, HI Kilic, A Suner

Abstract


Methicillin-resistant Staphylococcus aureus (MRSA) is a nasal infectious pathogen which is becoming of significant importance year by year. Mortality, morbidity and treatment costs of MRSA infections
have all increased. The most effective preventative tool is rapid confirmation of MRSA existence, followed by efficient execution of the required infection control measures. This study was performed
with the aim of evaluating MRSA colonization in health care staff from intensive care units (internal and surgical intensive care units) (ICUs) and how certain risk factors affect their colonization status. The study was conducted prospectively using samples obtained from nasal swabs of health-care staffs working in different missions in the intensive care unit of Gaziantep University Training Hospital in southeast of Turkey. The nasal swab samples were processed using a real-time PCR method platform
called GeneXpert (Cepheid). Our PCR screen revealed the presence of MRSA in 14 of 98 health-care staffs. Of these 14 health-care staffs carrying nasal MRSA, 10 were male, 8 were assistant health-care personnel and 11 have been working for over one year in the intensive care unit. Our data showed that male gender and an employment during of more than one year served as significant risk factors for nasal MRSA colonization.



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