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Mature embryos were used as explants. Five different concentrations of 2,4-D; 2, 2.5, 3, 3.5 and 4 mg/L were used for callus induction. For regeneration, initially different concentrations (0.1 to 0.2) of IAA
(indole-3-acetic acid) and BAP (6-benzylaminopurine) were experimented. The best combination of these hormones that is, 0.1 mg/L IAA and 0.5 mg/L BAP were further subjected to experimentation along with different concentrations of kinetin; 0.1, 0.2, 0.3, 0.4, 0.5 and 1 mg/L. Maximum calli of Kohsar (83.3%) was obtained at 3 mg/L 2,4-D whereas for Khyber-87 maximum callus induction (71.70%) was obtained at 3.5 mg/l 2,4-D. The maximum regeneration of both Kohsar and Khyber-87 (80.5 and 62.2%, respectively) were obtained at the combinations of 0.1 mg/L IAA, 0.5 mg/L BAP and 0.5 mg/L kinetin.