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African Journal of Biotechnology

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Study on genetic variability of Cassidula aurisfelis (snail) by random amplified polymorphic DNAs

W Bayani, WO Mohammad, S Shafie, K Zaleha

Abstract


The genetic variability among individuals of Cassidula aurisfelis from Setiu Wetland, Terengganu Darul Iman was examined by using the random amplified polymorphic DNA (RAPD) technique. Ten oligonucleotide primers were screened and three primers were selected (OPA 02, OPA 04 and OPA 10)
to amplify DNA from five samples of C. aurisfelis from Setiu Wetland, Terengganu. A total of 28 RAPD bands (RAPDs) with 17 polymorphic bands (60.71%) with size ranging from 300 – 1750 bp were scored from the population. Genetic distance for samples ranges from 0.135 to 0.269. Similarity index of samples ranges from 0.7179 to 0.8649 (mean 0.7810 ± 0.0497).



http://dx.doi.org/10.5897/AJB2009.000-9471
AJOL African Journals Online