African Journal of Biotechnology

The AJOL site is currently undergoing a major upgrade, and there will temporarily be some restrictions to the available functionality.
-- Users will not be able to register or log in during this period.
-- Full text (PDF) downloads of Open Access journal articles will be available as always.
-- Full text (PDF) downloads of subscription based journal articles will NOT be available
We apologise for any inconvenience caused. Please check back soon, as we will revert to usual policy as soon as possible.

Evaluation of wheat by polyacrylamide gel electrophoresis

M Shuaib, M Jamal, H Akbar, I Khan, R Khalid


In this study 15 wheat varieties namely Nacozari-76, Ouqab, Tatar-96, Bakhtawar-92, Yecura-70, Raj, Bakkar, Sulilman-96, Pirsabak-05, Maria, Khyber-87, Fakhr-e-Sarhad, Pirsabak-04, Inqulab-91 and Rawal-
87 were evaluated for analysis of variability in seed storage proteins by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Electrophorogram for each variety were scored and presence or absence of each band noted and was entered in a binary data matrix. Based on the data of SDS-PAGE gels cluster analysis was performed to check the variations among varieties. The overall result shows low degree of heterogeneity however different varieties reveal differential protein banding pattern. It is concluded that SDS-PAGE analysis of wheat endosperm protein is useful for evaluation of genetic variability and cultivars identification that help in wheat breeding program.

AJOL African Journals Online