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African Journal of Biotechnology

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Evaluation of wheat by polyacrylamide gel electrophoresis

M Shuaib, M Jamal, H Akbar, I Khan, R Khalid

Abstract


In this study 15 wheat varieties namely Nacozari-76, Ouqab, Tatar-96, Bakhtawar-92, Yecura-70, Raj, Bakkar, Sulilman-96, Pirsabak-05, Maria, Khyber-87, Fakhr-e-Sarhad, Pirsabak-04, Inqulab-91 and Rawal-
87 were evaluated for analysis of variability in seed storage proteins by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Electrophorogram for each variety were scored and presence or absence of each band noted and was entered in a binary data matrix. Based on the data of SDS-PAGE gels cluster analysis was performed to check the variations among varieties. The overall result shows low degree of heterogeneity however different varieties reveal differential protein banding pattern. It is concluded that SDS-PAGE analysis of wheat endosperm protein is useful for evaluation of genetic variability and cultivars identification that help in wheat breeding program.



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