Main Article Content

Molecular cloning and characterization of a novel expressed sequence tag (EST) associated with fecundity in goats


YQ He
J Kong
XK Ma
CX Zhang
XY Liu
HQ Chen

Abstract

To screen the genes controlling the fecundity traits in goats, a DDRT-PCR technique was applied. We found a new EST which highly expressed in Chinese native prolific goat breed, Haimen goats. There exists a difference of EST expression level between the prolific and non-prolific goat breed, indicating EST might associate to fecundity in goats. A full-length cDNA with 2253 base pairs was obtained by the 3’- and 5’-RACE method based on the EST sequence encoding a protein segment of 201 amino acid residues. Tissue specific distribution and sequence analysis implicated the likely involvement of EST in the regulation of the hormones related to fecundity.

Journal Identifiers


eISSN: 1684-5315