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DOI:
10.5897/AJB09.1722Keywords:
Lentiviral vector coagulation factor VIII transfection in vitro gene therapy
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Selected cultivars of cornelian cherry (<i>Cornus mas</i> L.) as a new food source for human nutrition
Abstract
The aim of this work was to determine antioxidant activity in 12 cultivars of cornelian cherry (Cornus
mas L.). Two assays based on ion reduction of ABTS (2,2´-azinobis(3-ethylbenzothiazoline-6-
sulphonate) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals were used for antioxidant activity. Total
phenolic content of the fruit was analysed by Folin-Ciocelteau colorimetric method and ascorbic acid
content was analysed using column chromatography - electrochemical detector (Coulochem III). The
highest amounts of total phenolic content were found in cultivars ´Vydubeckij´ and ´Titus´ as 7.96 and
8.11 g gallic acid kg-1 of fresh mass (FM). High correlation between polyphenols and antioxidant activity
in fruits of the cultivars was observed (r2 = 0.970 for DPPH test and r2 = 0.978 for ABTS test). The
highest total content of ascorbic acid was determined in cultivar ´Olomoucky´, with the value of 3.11 g
kg-1 FM. This study attempts to contribute to the knowledge of human nutritional properties of these
cornelian cherry cultivars and may be useful for the evaluation of dietary information and further
propagation of cultivation and utilization of this fruit in the world.
mas L.). Two assays based on ion reduction of ABTS (2,2´-azinobis(3-ethylbenzothiazoline-6-
sulphonate) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals were used for antioxidant activity. Total
phenolic content of the fruit was analysed by Folin-Ciocelteau colorimetric method and ascorbic acid
content was analysed using column chromatography - electrochemical detector (Coulochem III). The
highest amounts of total phenolic content were found in cultivars ´Vydubeckij´ and ´Titus´ as 7.96 and
8.11 g gallic acid kg-1 of fresh mass (FM). High correlation between polyphenols and antioxidant activity
in fruits of the cultivars was observed (r2 = 0.970 for DPPH test and r2 = 0.978 for ABTS test). The
highest total content of ascorbic acid was determined in cultivar ´Olomoucky´, with the value of 3.11 g
kg-1 FM. This study attempts to contribute to the knowledge of human nutritional properties of these
cornelian cherry cultivars and may be useful for the evaluation of dietary information and further
propagation of cultivation and utilization of this fruit in the world.
