PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Biotechnology

Log in or Register to get access to full text downloads.

Remember me or Register



Protective effect of nitric oxide against arsenic-induced oxidative damage in tall fescue leaves

JW Jin, YF Xu, YF Huang

Abstract


Nitric oxide (NO) is a key molecule involved in many physiology processes. The effects of NO on alleviating arsenic-induced oxidative damage in tall fescue leaves were investigated. Arsenic (25 M) treatment induced significantly accumulation of reactive oxygen species (ROS) and led to serious lipid peroxidation in tall fescue leaves and the application of 100 M SNP before arsenic stress resulted in alleviated arsenic-induced electrolyte leakage and malondiadehyde (MDA) content in tall fescue leaves, the levels of hydrogen peroxide (H2O2) and superoxide radical (O2̅ ) were reduced as well. Moreover, the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) increased in tall fescue leaves in presence of SNP under arsenic stress. This pattern was reversed by application of NO scavenger, 2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethy-limidazoline-1-oxyl-3-oxide (PTIO) before arsenic treatment. Pronounced increases in endogenous NO production was found in plants after exposure to arsenic stress. The results suggested that arsenic stress elevated endogenous NO level and that NO might act as a signaling molecule to enhance antioxidant enzyme activities, further protecting against injuries caused by arsenic toxicity.



http://dx.doi.org/10.5897/AJB10.1442
AJOL African Journals Online