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African Journal of Biotechnology

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Assessment of genetic and biochemical diversity of ecologically variant ectomycorrhizal Russula sp. from India

MK Upadhyay, D Jain, A Singh, AK Pandey, RC Rajak

Abstract


The aim of this study was to develop the phylogenetic relationship amongst the ecologically variant Russula species by using polymerase chain reaction (PCR) based technique, random amplified polymorphic DNA (RAPD) and isozyme analyses. Two groups could be characterized amongst the total isolates by cluster analyses. Protease, cellulase, glutamate dehydrogenase,  pectinase and acid phosphatase designated band P220.16, C472.18, GLD130.21, Pe569.12 and AP472.12, respectively, were common in all the isolates and four monomorphic RAPD bands viz; 818, 512, 298 and 201 bp were also diversified in the isolates. This common band reveals that diversity of these alleles or loci in all ecologically variant isolates. Thus, the present studies discuss the genetic diversity of ecologically variant Russula species on the basis of RAPD and isozyme analysis.



http://dx.doi.org/10.5897/AJB10.1770
AJOL African Journals Online