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insecticide for centuries in traditional Croatian farming systems. It has no toxicity to man and animals but possesses ecological benefits that have led to increasing worldwide production of this natural insecticide. Nowadays, it is cultivated mainly at higher altitudes in tropical countries such as Kenya, Tanzania and Rwanda. The present investigation was directed in identifying a simple and reliable extraction treatment using solvents with lower cost and toxicity and an adequate method for the identification and separation of active compounds (pyrethrins) with possible application in enterprises or industry. Best developed method was used for determination of pyrethrin content in three different natural populations of Chrysanthemum. The results revealed high content of total pyrethrins in
populations grown in Croatia. Developed method and good quality product give a possibility for this culture to become again an exporting and economically valid product for Croatia.