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acetonitrile and ammonium acetate buffer (pH 10.0). The detection wavelength was 240 nm. Intra-assay and inter-assay precision of the analyses were less than 10% and the average recovery rates obtained
were in the range of 85.63 - 90.94% for all analysis of the three aconitum alkaloids with relative standard deviations (RSD) below 14%. Positive linear relationships were observed in correlation coefficients that exceeded 0.95. The limits of detection (signal-to-noise ratio of 3) were 2.64, 1.58 and 2.75 ng for aconitine, mesaconitine and hypaconitine, respectively. The method can provide a scientific and technical platform to determine the concentration of aconitum alkaloids in plasma during a pilot pharmacokinetic study in rats.