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African Journal of Biotechnology

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A new protocol for extraction of C0t-1 DNA from rice

C Yan, G Li, L Hong, L Gang, Q Rui

Abstract


C<i>0</i>t-1 DNA, enriched for repetitive DNA sequences, has been proved to be valuable in the studies of plant species differentiation and genome evolution. A new protocol to steadily obtain the aimed range of DNA fragments has been developed by shearing the genomic DNA with the digest system containing DNase and DNA polymerase . Comparison through fluorescence in situ hybridization (FISH) - Oryza sativa L with labeled probe of C0t-1 DNA isolated by the autoclave equipment and the new procedure
was carried out. FISH result shows that the new procedure can be a feasible method to steadily isolate high-quality C<i>0</i>t-1 DNA.



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