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African Journal of Biotechnology

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In vitro salt stress induced production of gymnemic acid in callus cultures of Gymnema sylvestre R.Br.

U Kumar, I Singh, P Priyanka, Y Vimala

Abstract


Gymnema sylvestre, a well known antidiabetic plant, was cultured in vitro for callus induction using leaf explant. Gymnemic acid content enhancement under the influence of different plant hormones like 2,4- D, BA with or without NaCl supplementation to the MS medium is presented in this investigation. The highest efficiency of callus formation was observed in the medium containing different concentrations of 2,4-D. The gymnemic acid content increased with increasing concentration of 2,4-D along with NaCl.

Key words: In vitro, Gymnema sylvestre, callus culture, gymnemic acid, salt stress.




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