In vitro salt stress induced production of gymnemic acid in callus cultures of Gymnema sylvestre R.Br.

Abstract

Gymnema sylvestre, a well known antidiabetic plant, was cultured in vitro for callus induction using leaf explant. Gymnemic acid content enhancement under the influence of different plant hormones like 2,4- D, BA with or without NaCl supplementation to the MS medium is presented in this investigation. The highest efficiency of callus formation was observed in the medium containing different concentrations of 2,4-D. The gymnemic acid content increased with increasing concentration of 2,4-D along with NaCl.

Key words: In vitro, Gymnema sylvestre, callus culture, gymnemic acid, salt stress.