African Journal of Biotechnology

The AJOL site is currently undergoing a major upgrade, and there will temporarily be some restrictions to the available functionality.
-- Users will not be able to register or log in during this period.
-- Full text (PDF) downloads of Open Access journal articles will be available as always.
-- Full text (PDF) downloads of subscription based journal articles will NOT be available
We apologise for any inconvenience caused. Please check back soon, as we will revert to usual policy as soon as possible.

Genomic study of the cereolysin A and B genes in Bacillus cereus isolated from raw and pasteurized milk

S Fallah, M Chamani, M Aminafshar, H Ezzatpanah


Bacillus cereus spores are expanded in the nature and they can be separated from different foods. Cytotoxin is one of the most important poisons which is produced by Bacillus cereus, and it is highly resistant to heat and leads to diarrhea, nausea and vomiting syndrome. Hence study about existence of Bacillus cereus in pasteurized milk is very important due to probability of causing illness by Cereolysin gene products. Therefore, Different milk samples were collected from raw milk to pasteurized milk after various stages of producing pasteurized milk. Cultivation of milk samples in Mannitol egg yolk polymyxin B (MYP) media was done and it was followed by a purification of the observed colonies and
investigation of cereolysin A and B by polymerase chain reaction (PCR) amplification. Thus, the gene was amplified and aligned with the previous sequences that were registered in the gene bank database. As such, many new missense mutations were observed at the amplified gene sequences. However these missense mutations caused a change in the sequence of amino acid at the protein chain but the protein efficiency and structure was not changed due to the substitution of amino acids with the same

Key words: milk- cereolysin - Bacillus cereus- polymerase chain reaction- gene.

AJOL African Journals Online