Application of genefishing discovery system on differential gene expression study for prokaryotic system

  • KH Chua
  • HC Chai
  • SD Puthucheary

Abstract

GeneFishing Discovery is a system applied on disease and gene related research for eukaryotes and the starting material for the study could be either total RNA or mRNA. In this study we demonstrate the use of this discovery system for a prokaryotic system by modifying the eukaryotic protocol using the poly (A)-tailing approach. As an example we compared the gene expression pattern of two closely related species of bacteria under the same family (Burkholderia thailandensis and Burkholderia cepacia) with satisfactory results.

Keywords: Genefishing, differential expression, prokaryotic system

African Journal of Biotechnology Vol. 9(35), pp. 5629-5631, 30 August, 2010

Author Biographies

KH Chua
Departments of Molecular Medicine University of Malaya, 50603 Kuala Lumpur, Malaysia
HC Chai
Departments of Molecular Medicine University of Malaya, 50603 Kuala Lumpur, Malaysia
SD Puthucheary

Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia

Published
2013-08-16
Section
Articles

Journal Identifiers


eISSN: 1684-5315