Evaluation of substrates from renewable-resources in biosurfactants production by Pseudomonas strains

  • Sidnei Cerqueira dos Santos
  • Luzimar Gonzaga Fernandez
  • Juan Carlos Rossi-Alva
  • Milton Ricardo de Abreu Roque

Abstract

The aim of the present study is to evaluate biosurfactant production by two strains of Pseudomonas spp. using alternative carbon sources. The bacterial strains used were isolated from the Municipal Park of Mucugê, Bahia State, Brazil. Different vegetable oils and glycerol concentrations were evaluated and used individually, as the sole source of carbon and energy for the production of biosurfactant which was measured by the test of emulsification and surface tension. The best concentration of alternative substrates achieved was 0.5%, with emulsion formation rates ranging from 50 to 59% for both bacterial strains analyzed. The free-cell broth of Pseudomonas  strains had the ability to reduce the surface tension of all culture media tested, although it was more effective when glycerol was used. The results confirm the potential of these alternative substrates, based on the availability and low cost of these raw materials, to reduce the final production cost and also the expenses with waste treatment.

Keywords: Pseudomonas, biosurfactants, substrates, renewable-resources

African Journal of Biotechnology Vol. 9(35), pp. 5704-5711, 30 August, 2010

Author Biographies

Sidnei Cerqueira dos Santos
Programa de Pós-Graduação em Biotecnologia, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Bahia, Brazil; Laboratório de Biotecnologia e Ecologia de Microrganismos, Departamento de Biointeração, Universidade Federal da Bahia, Bahia, Brazil
Luzimar Gonzaga Fernandez
Laboratório de Bioquímica, Biotecnologia e Bioenergia, Departamento de Biofunção, Universidade Federal da Bahia, Bahia, Brazil
Juan Carlos Rossi-Alva
Laboratório de Estudos em Meio Ambiente, Superintendência de Pesquisa e Pós-Graduação, Universidade Católica do Salvador, Bahia, Brazil
Milton Ricardo de Abreu Roque
Programa de Pós-Graduação em Biotecnologia, Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Bahia, Brazil; Laboratório de Biotecnologia e Ecologia de Microrganismos, Departamento de Biointeração, Universidade Federal da Bahia, Bahia, Brazil
Published
2013-08-18
Section
Articles

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eISSN: 1684-5315