A potato NOA gene increased salinity tolerance in Arabidopsis thaliana

  • B Zhang
  • HQ Wang
  • BL Liu
  • J Liu
  • X Wang
  • Q Liu
  • HG Zhang

Abstract

The purpose of this study is to produce recombinant StNOA1 in transgenic plants and to test its potential role in plant salt stress responses. The newly cloned StNOA1 gene from Solanum tuberosum L. was inserted into AtnOA1 mutant plant genome by Agrobaterium-mediated floral dip method. Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenic Atnoa1. Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR. Physiological analyses indicated that the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition. Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress. Present study proved that StNOA1 participated in Arabidopsis thaliana salt stress responses and increased its salinity tolerance.

Keywords: StNOA1 transformation, Solanum tuberosum, Atnoa1 mutant, salt tolerance

African Journal of Biotechnology Vol. 9(36), pp. 5869-5878, 6 September, 2010

Author Biographies

B Zhang
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P.R. China; Graduate University of Chinese Academy of Sciences, Beijing, 100049, P.R. China
HQ Wang
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P.R. China
BL Liu
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P.R. China
J Liu
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P.R. China; Graduate University of Chinese Academy of Sciences, Beijing, 100049, P.R. China
X Wang
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P.R. China; Graduate University of Chinese Academy of Sciences, Beijing, 100049, P.R. China
Q Liu
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P.R. China; Graduate University of Chinese Academy of Sciences, Beijing, 100049, P.R. China
HG Zhang
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P.R. China
Published
2013-08-21
Section
Articles

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