Isolation and molecular characterization of RcSERK1: A Rosa canina gene transcriptionally induced during initiation of protocorm-like bodies
A somatic embryogensis receptor-like kinase (SERK) gene was isolated from protocorm-like bodies (PLBs) of Rosa canina by a rapid amplification of cDNA ends (RACE) approach and was designated as RcSERK1. The RcSERK1 encodes a protein of 626 amino acid residues with a calculated molecular mass of 68.79 kDa and theoretical isoelectric point of 5.65. The amino acid sequence of RcSERK1 shares all the characteristic features of a SERK protein, including the signal peptide (SP), the leucine zipper (LZ), the five leucine-rich repeats (LRRs), the pro-rich domain containing the so-called Ser-Pro- Pro (SPP) motif, the transmembrane domain (TM), the kinase domain and the C-terminal domain. The transcripts of RcSERK1 were more enriched in PLBs than in rhizoids and callus, but not detected in leaflets (incubated under dark and before producing callus) and the regenerated shoots. Subcellular localization indicated that the fluorescence of RcSERK1-GFP was recorded in the plasma membrane. We argue that RcSERK1 is a Leu-rich repeat receptor-like kinase (LRR-RLK) and plasma membrane localization protein.
Keywords: somatic embryogensis receptor-like kinase (SERK)1, protocorm-like bodies (PLBs), Rosa canina, RACE, RcSERK1.