Optimization of a plant regeneration protocol for broccoli

Abstract

The factors which influence the regeneration of broccoli (Brassica oleracea var. italica) were studied using an orthogonal design. The results showed that the major factor was the explant type, followed by naphthylacetic acid (NAA), benzylaminopurine (BAP), sucrose and AgNO₃ in turn. The maximum regeneration was on Murashige and Skoog (MS) medium + NAA 0.107 μM + BAP 17.76 μM + 2% sucrose + 0.8% agar. Hypocotyl proved to be the optimum explant source and its regeneration frequency reached 100%.

Key words: Naphthylacetic acid (NAA), benzylaminopurine (BAP), AgNO3, hypocotyl, broccoli (Brassica oleracea var. italica).