Comparisons of stomatal parameters between normal and abnormal leaf of <i>Bougainvillea spectabilis</i> Willd

Abstract

In this study, stomatal density (SD), stomatal index (SI), epidermal cell density (ED), guard cell length (GCL) and guard cell width (GCW) of normal and abnormal leaf of Bougainvillea spectabilis Willd were studied. This can be useful for further research of physical mechanism of abnormal leaf. Epidermal cells were polygonal with straight anticlinal walls in the adaxial epidermis of normal leaf; while in the abaxial epidermis of normal leaf, the epidermal cells were irregular with repand anticlinal walls. The stomata were anomocytic on normal leaf. No stomata were found in the adaxial epidermis of abnormal leaf. In abaxial epidermis of abnormal leaf, the epidermal cells were repand or sinuous. The stomata were anomocytic on abnormal leaf. The guard cells were kidney-shaped on normal and abnormal leaf. SD and SI of normal leaf were significantly higher than that of abnormal leaf. ED and GCL of abnormal leaf were higher than that of normal leaf in relative leaves length 37.5 and 50%. At other four stages, ED and GCL of abnormal leaf were smaller than that of normal leaf. SD, SI and ED had a negative correlation with relative leaf length; a significant positive correlation was observed between GCL and relative leaf length.

Key words: Bougainvillea spectabilis Willd, normal leaf, abnormal leaf, stomatal density, epidermal cell density, stomatal index.