A novel missense mutation of bovine lipase maturation factor 1 (LMF1) gene and its association with growth traits

  • G Ren
  • JX Liu
  • F Li
  • XY Lan
  • MJ Li
  • ZY Zhang
  • H Chen
Keywords: Bovine, polymorphism, growth traits, LMF1 gene.


Lipase maturation factor 1 (LMF1) gene is a novel candidate gene in severe hypertriglyceridemia. To detect the polymorphism in LMF1 gene in 804 Chinese cattle, we firstly described the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP), DNA sequence and PCR-RFLP methods for detecting mutations of LMF1 gene, which affected growth and development in animal. The results showed a novel mutation in exon 4: NC_007326.3: g.27154 T>C, which resulted in a missense mutation within LMF1 protein (p.197 Trp>Arg). Genotype TT was dominant in four breeds, and genotypic frequencies of LMF1 exon 4 AvaI locus were calculated in four populations which agreed with Hardy- Weinberg equilibrium (p > 0.05). The association analysis showed that individuals with genotype TC had greater body weight than those with genotype TT at 12, 18 and 24 months of age in Nanyang cattle (P < 0.05).

Key words: Bovine, polymorphism, growth traits, LMF1 gene.


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eISSN: 1684-5315